Loss of heterozygosity for YNZ22 loci in human ovarian carcinomas

objective To detect the loss of heterozygosity(LOH) for YNZ22 loci in human ovarian carcinomas,and discuss the relationship between LOH and clinical pathology.Methods To analyse amplified fragment length polymorphism(Amp FLP) at locus YNZ22 of cancer and normal tissues in 30 cases with ovarian carcinomas by using PCR followed by PAGE and silver staining.Results The allele frequencies and heterozygosity were determined.Of 23 informative cases,the heterozygosity was 76.7%,the LOH was 13.0%.Conclusion The loss of heterozygosity for YNZ22 loci in ovarian carcinomas is related to the differentiation degrees and clinical stages.\;