子宫内膜
H&E染色
细胞角蛋白
免疫组织化学
子宫
男科
染色
生理盐水
波形蛋白
内分泌学
内科学
化学
生物
医学
病理
出处
期刊:Life Science Research
日期:2011-01-01
摘要
To explore a novel method to establish a thin endometrium by injecting ethanol into the uterine cavity of rat.Twenty-five female SD rats were divided into three groups: control group(physiological saline were injected into cavities and kept for 5 min),5 minutes group(95% ethanol was injected and kept for 5 min) and 10 minutes group(95% ethanol was injected and kept for 10 min).The endometria which were thin-ner than that of the control group would be taken as a new group-model group,the others were control group.Observe endometrial morphology by hematoxylin-eosin staining(HE) and analyze the growth of the epithelial and stroma cells,VEGF and ERα expression by immunohistochemical staining method.Fourteen u-terine endometria thinned in 5 minutes group and the endometrial layers even all uterine layers of 16 uteri were necrosed in 10 minutes group.All endometria were characterized by poor regrowth in model group.Compared with control group,the cytokeratin and the vimentin area per unit endometrium area was less(P0.05);VEGF expressed lower in ndometrium(P0.05);the expression of ERα was the same(P0.05) between two groups.A rat model of thin endometrium was successfully established when 95% ethyl alcohol was kept in uterine cavities for 5 min,and the success rate was 70%.
科研通智能强力驱动
Strongly Powered by AbleSci AI