PENGARUH FLAVONOID RUTIN TERHADAP EFEK ANALGETIK DAN HEPATOTOKSISITAS PARASETAMOL

Flavonoid is a secondary metabolite produced by plants. In plants, flavonoid can be found as aglycone from glycoside. For example, flavonoid quercetin is an aglycone from glycoside rutin. Quercetin has free hydroxyl groups which have been proven can inhibit cytochrome P450. Rutin, as a glycoside of quercetin is assumed having similiar activity. One of many drugs which metabolism is catalyzed by P450 is paracetamol. This research aimed to obtain the relation between rutin towards analgesic activity and hepatotoxicity of paracetamol. This study used male mice from Balb/C strain (Mus musculus). They were divided randomly into groups of two kinds of treatment, with and without rutin. Both groups were observed upon analgesic and hepatotoxicity test. As for treatment with rutin, mice were given rutin suspension in CMC 0,5% orally once per day for seven days and followed by paracetamol. Dosage of rutin used in this study was 10 mg/kgBW and 40 mg/kgBW. Analgesic activity was measured with its percentage, while hepatotoxicity was observed through liver organ histopathology based on abnormality of tissue structure. The result was analyzed by Kolmogorov Smirnov, ANOVA, and LSD tests with confidence level of 95%. This study showed that treatment of 10 mg/kgBW and 40 mg/kgBW rutin increased analgesic activity of paracetamol. However only at 40 mg/kgBW treatment gave significant result compared to control. The significant effect could be obtained at 91 mg/kgBW therapeutic dose and also at 300 mg/kgBW toxic dose. Analgesic activity of paracetamol is proportional to the increase in rutin dosage given . Furthermore the hepatotoxicity test showed 10 mg/kgBW or 40 mg/kgBW rutin could not decrease hepatotoxicity that caused by paracetamol at toxic dose.