Production optimization of pullulanase enzyme produced by Bacillus cereus isolated from Syrian sources

The objective of this study was to isolate, identification the highest pullulanase that produced by Syrian bacteria from different environmental sources such as baking wastes, soil, and food wastes samples, then optimization production of pullulanase. 17 isolates was positive for pullulanase production, The isolate P8 demonstrated the highest extracellular pullulanase activity, it was identified as Bacillus cereus based on Morphological and biochemical experiments. Culture conditions parameters for production enzyme were optimized partial characterization of extracellular pullulanase from Bacillus cereus. The results showed maximum pullulanase production occurred after 48 h, 10 7 colony frame unit (cfu), at pH 7.5. The optimum temperature for pullulanase production was found to be 37°C in the presence of 1% soluble pullulan as a carbon source and 0.5% tryptone. The activity of pullulanse at this optimal conditions was (1.18 U/ml) which is 2.14 fold higher than before optimization. By characterization of pullulanase, highest activity observed at pH 6.0 and 50°C.