Targeting intracellular WT1 in AML with a novel RMF-peptide-MHC-specific T-cell bispecific antibody

抗原 T细胞 细胞毒性 抗体 癌症研究 免疫疗法 表位 分子生物学 生物 免疫学 体外 免疫系统 生物化学
作者
Christian Augsberger,Gerulf Hänel,Wei Xu,Vesna Pulko,Lydia Jasmin Hanisch,Angélique Augustin,John Challier,Katharina Hunt,Binje Vick,Pier Edoardo Rovatti,Christina Krupka,Maurine Rothe,Anne Schönle,Johannes Sam,Emmanuelle Lezan,Axel Ducret,Daniela Ortiz Franyuti,Antje-Christine Walz,Jörg Benz,Alexander Bujotzek,Felix S. Lichtenegger,Christian Gassner,Alejandro Carpy,Victor I. Lyamichev,Jigar Patel,Nikola P. Konstandin,Antje Tunger,Marc Schmitz,Michael von Bergwelt‐Baildon,Karsten Spiekermann,Luca Vago,Irmela Jeremias,Estelle Marrer-Berger,Pablo Umaña,Christian Klein,Marion Subklewe
出处
期刊:Blood [American Society of Hematology]
卷期号:138 (25): 2655-2669 被引量:42
标识
DOI:10.1182/blood.2020010477
摘要

Abstract Antibody-based immunotherapy is a promising strategy for targeting chemoresistant leukemic cells. However, classical antibody-based approaches are restricted to targeting lineage-specific cell surface antigens. By targeting intracellular antigens, a large number of other leukemia-associated targets would become accessible. In this study, we evaluated a novel T-cell bispecific (TCB) antibody, generated by using CrossMAb and knob-into-holes technology, containing a bivalent T-cell receptor–like binding domain that recognizes the RMFPNAPYL peptide derived from the intracellular tumor antigen Wilms tumor protein (WT1) in the context of HLA-A*02. Binding to CD3ε recruits T cells irrespective of their T-cell receptor specificity. WT1-TCB elicited antibody-mediated T-cell cytotoxicity against AML cell lines in a WT1- and HLA-restricted manner. Specific lysis of primary acute myeloid leukemia (AML) cells was mediated in ex vivo long-term cocultures by using allogeneic (mean ± standard error of the mean [SEM] specific lysis, 67 ± 6% after 13-14 days; n = 18) or autologous, patient-derived T cells (mean ± SEM specific lysis, 54 ± 12% after 11-14 days; n = 8). WT1-TCB–treated T cells exhibited higher cytotoxicity against primary AML cells than an HLA-A*02 RMF-specific T-cell clone. Combining WT1-TCB with the immunomodulatory drug lenalidomide further enhanced antibody-mediated T-cell cytotoxicity against primary AML cells (mean ± SEM specific lysis on days 3-4, 45.4 ± 9.0% vs 70.8 ± 8.3%; P = .015; n = 9-10). In vivo, WT1-TCB–treated humanized mice bearing SKM-1 tumors exhibited a significant and dose-dependent reduction in tumor growth. In summary, we show that WT1-TCB facilitates potent in vitro, ex vivo, and in vivo killing of AML cell lines and primary AML cells; these results led to the initiation of a phase 1 trial in patients with relapsed/refractory AML (#NCT04580121).
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