生物传感器
费斯特共振能量转移
滚动圆复制
纳米技术
量子点
DNA
化学
多路复用
环介导等温扩增
荧光
材料科学
计算机科学
物理
生物化学
聚合酶
电信
量子力学
作者
Jingyue Xu,Xue Qiu,Niko Hildebrandt
出处
期刊:Nano Letters
[American Chemical Society]
日期:2021-05-27
卷期号:21 (11): 4802-4808
被引量:19
标识
DOI:10.1021/acs.nanolett.1c01351
摘要
Isothermal nucleic acid amplification strategies have been combined with nanotechnology for advanced biosensing, material design, and biomedical applications. However, merging phenomena and materials of different nanoscales with the aim of exploiting all their benefits at once has remained a challenging endeavor. Here, we exemplify the various problems one can encounter when combining the nanodimensions of lanthanide complexes (∼2 nm), Förster resonance energy transfer (FRET, ∼5 nm), quantum dots (QDs, ∼20 nm), and rolling circle amplification (RCA, ∼250 nm) into a single microRNA biosensor and how these challenges can be overcome. Six different approaches, including simple FRET-RCA, enzyme-digesting FRET-RCA, and FRET-hyperbranched-RCA were investigated. We demonstrated specific miR-21 detection with 80 fM limit of detection and multiplexing capability with FRET from a Tb complex to different QDs. The detailed view on the various complex multi-nanodimensional assay systems elucidated the limited clinical translation of such sophisticated multicomponent nanobiosensors.
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