Multichannel silk protein/laminin grafts for spinal cord injury repair

Journal of Biomedical Materials Research Part AVolume 104, Issue 12 p. 3045-3057 Original Article Multichannel silk protein/laminin grafts for spinal cord injury repair Qiang Zhang, Qiang Zhang National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts These authors contributed equally to this work.Search for more papers by this authorShuqin Yan, Shuqin Yan National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China These authors contributed equally to this work.Search for more papers by this authorRenchuan You, Renchuan You National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 ChinaSearch for more papers by this authorDavid L. Kaplan, David L. Kaplan Department of Biomedical Engineering, Tufts University, Medford, 02155 MassachusettsSearch for more papers by this authorYu Liu, Yu Liu National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaSearch for more papers by this authorJing Qu, Jing Qu National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaSearch for more papers by this authorXiufang Li, Xiufang Li National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaSearch for more papers by this authorMingzhong Li, Corresponding Author Mingzhong Li mzli@suda.edu.cn National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaCorrespondence to: M. Li; e-mail: mzli@suda.edu.cnSearch for more papers by this authorXin Wang, Xin Wang Centre for Advanced Materials and Performance Textiles, School of Fashion and Textiles, RMIT University, Melbourne, 3056 Victoria, AustraliaSearch for more papers by this author Qiang Zhang, Qiang Zhang National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts These authors contributed equally to this work.Search for more papers by this authorShuqin Yan, Shuqin Yan National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China These authors contributed equally to this work.Search for more papers by this authorRenchuan You, Renchuan You National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 ChinaSearch for more papers by this authorDavid L. Kaplan, David L. Kaplan Department of Biomedical Engineering, Tufts University, Medford, 02155 MassachusettsSearch for more papers by this authorYu Liu, Yu Liu National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaSearch for more papers by this authorJing Qu, Jing Qu National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaSearch for more papers by this authorXiufang Li, Xiufang Li National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaSearch for more papers by this authorMingzhong Li, Corresponding Author Mingzhong Li mzli@suda.edu.cn National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 ChinaCorrespondence to: M. Li; e-mail: mzli@suda.edu.cnSearch for more papers by this authorXin Wang, Xin Wang Centre for Advanced Materials and Performance Textiles, School of Fashion and Textiles, RMIT University, Melbourne, 3056 Victoria, AustraliaSearch for more papers by this author First published: 30 July 2016 https://doi.org/10.1002/jbm.a.35851Citations: 24 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The physical, chemical, and bioactive cues provided by biomaterials are critical for spinal cord regeneration following injury. In this study, we investigated the bioactivity of a silk-based scaffold for nerve tissue remodeling that featured morphological guidance in the form of ridges as well as bioactive molecules. Multichannel/laminin (LN) silk scaffolds stimulated growth, development, and the extension of primary hippocampal neurons after 7 days of culture in vitro. And then, the multichannel/LN silk scaffolds were implanted into 2-mm-long hemisection defects in Sprague-Dawley rat spinal cords for 70 days to evaluate their bioactivities of spinal cord remolding. Our results demonstrated that animal behavior was significantly improved in the multichannel/LN group, as evaluated by Basso–Beattie–Bresnahan score, whereas the implantation of multichannels and random pores groups resulted in recurring limps. Moreover, histology and immunohistochemical staining revealed an increase in blood vessels and expression of growth associated protein-43 and neurofilament-200 as well as reduced expression of glial fibrillary acidic protein in the multichannel/LN group, which contributed to the rebuilding of spinal cord defects. Thus, multichannel/LN silk scaffolds mediated cell migration, stimulated blood capillary formation, and promoted axonal extension, suggesting the utility of these scaffolds for spinal cord reconstruction. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3045–3057, 2016. Citing Literature Volume104, Issue12December 2016Pages 3045-3057 RelatedInformation