生物
电池类型
转录组
计算生物学
细胞
长非编码RNA
功能(生物学)
基因表达
基因
细胞生长
核糖核酸
细胞生物学
遗传学
作者
Gyeong Dae Kim,Sadahito Shin,Su Woong Jung,Hyunsu An,Sin Young Choi,Minho Eun,Chang‐Duk Jun,Sang‐Ho Lee,Jihwan Park
出处
期刊:Journal of The American Society of Nephrology
日期:2024-04-15
标识
DOI:10.1681/asn.0000000000000354
摘要
Background Accumulated evidence demonstrates that long non-coding RNAs (lncRNAs) regulate cell differentiation and homeostasis, influencing kidney aging and disease. Despite their versatility, the function of lncRNA remains poorly understood due to the lack of a reference map of lncRNA transcriptome in various cell types. Methods In this study, we employed a targeted single-cell RNA sequencing (scRNA-seq) method to enrich and characterize lncRNAs in individual cells. We applied this method to various mouse tissues, including normal and aged kidneys. Results Through tissue-specific clustering analysis, we identified cell type-specific lncRNAs that showed a high correlation with known cell-type marker genes. Furthermore, we constructed gene regulatory networks (GRNs) to explore the functional roles of differentially expressed lncRNAs in each cell type. In the kidney, we observed dynamic expression changes of lncRNAs during aging, with specific changes in glomerular cells. These cell type- and age-specific expression patterns of lncRNAs provide insights into their potential roles in regulating cellular processes, such as immune response and energy metabolism, during kidney aging. Conclusions Our study sheds light on the comprehensive landscape of lncRNA expression and function and provides a valuable resource for future analysis of lncRNAs (https://gist-fgl.github.io/sc-lncrna-atlas/).
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