化学
体内
体外
半胱氨酸
荧光
功能(生物学)
生物物理学
对偶(语法数字)
纳米技术
生物化学
细胞生物学
光学
艺术
物理
材料科学
生物技术
文学类
生物
酶
作者
Lipan Zhou,Terry Chien‐Jen Yang,Tingrui Zhang,Zhanqian Song,Guodong Feng
出处
期刊:Talanta
[Elsevier]
日期:2024-02-01
卷期号:: 125769-125769
标识
DOI:10.1016/j.talanta.2024.125769
摘要
A fluorescent probe of both colorimetric and ratiometric type for highly selective and sensitive detection of Cys (cysteine) is very important in biological analysis. In this work, a new colorimetric and ratiometric fluorescent probe ((E)-2-(2-(5-(4-(acryloyloxy)phenyl)furan-2-yl)vinyl)-3-methylbenzo[d]thiazol-3–ium iodide, LP-1) was designed and synthesized for the detection of Cys. The reaction mechanism of LP-1 toward Cys involves a conjugate addition reaction between Cys and the α,β-unsaturated carbonyl group, leading to the formation of an intermediate thioether, followed by intramolecular cyclization to produce the desired compounds LP-1-OH. At this point, the ICT process is activated, significantly increasing the fluorescence intensity of the molecules. Meanwhile, LP-1 is highly selective and sensitive to Cys identification under optimized experimental conditions. LP-1 shows a good linear relationship in the range of Cys concentration from 0.40 μM to 40 μM (R2 = 0.9942) and the limit of detection (LOD) of Cys is 0.19 μM. In addition, we have developed a simple, portable and low-cost smartphone-based high-sensitivity Cys detection method based on naked eye obvious color detection. LP-1 also has low cell toxicity and can be successfully used for biological imaging of Cys, suggesting that it is a promising biological application tool for Cys detection.
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