地衣芽孢杆菌
转录组
发酵
生物
基因
生物化学
食品科学
遗传学
细菌
基因表达
枯草芽孢杆菌
作者
Anying Ji,Xiujue Zheng,Chunxiang Wei,Ming Chen,Aimin Ma,Yongfeng Liu,Xuetuan Wei
标识
DOI:10.1093/jambio/lxad319
摘要
Abstract Aims Bacillus licheniformis AQ is an industrial strain with high production of AprE, which has great industrial application value. But, how to regulate the production of AprE in the process of industrial fermentation is still not completely clear. Therefore, it is important to understand the metabolic process of AprE production in the industrial fermentation medium. Methods and results In this study, transcriptome sequencing of the whole fermentation course was performed to explore the synthesis and regulation mechanism of AprE in B. licheniformis AQ. During the fermentation process, the AprE is continuously accumulated, reaching a peak of 42,020 U/mL at the fermentation endpoint (48 h). Meanwhile, the highly expressed genes were observed. Compared with the fermentation endpoint, there are 61 genes in the intersection of differentially expressed genes, functioning as catabolic processes, peptidases and inhibitors, chaperones and folding catalysts. Furthermore, the protein-protein interactions network of AprE was constructed. Conclusion This study provides important transcriptome information for B. licheniformis AQ and potential molecular targets for further improving the production of AprE.
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