肺炎克雷伯菌
免疫原性
微生物学
肺炎
免疫系统
病菌
肺炎克雷伯菌
免疫
细菌外膜
细菌
医学
生物
免疫学
铜绿假单胞菌
大肠杆菌
基因
生物化学
遗传学
内科学
作者
Lai Jiang,Linyu Ding,Qiuyue Long,Xuan Li,Jiwei Li,Jingjiao Ma,Yali Zheng,Gang Liu
出处
期刊:Nano Today
[Elsevier]
日期:2024-02-01
卷期号:54: 102135-102135
标识
DOI:10.1016/j.nantod.2023.102135
摘要
Klebsiella pneumoniae is the main pathogen of hospital-acquired infections causing acute pneumonia, which is characterized by drug resistance, virulence and mortality, and for which there is no clinically approved vaccine formulations. Here, we designed biomimetic hybrid membrane vesicles (HMVs), which consist of outer membrane vesicles of Klebsiella pneumoniae and cell membrane vesicles of alveolar macrophages. The HMVs were administered via intratracheal instillation to mimic the delivery of an inhaled formulation, where immune cells could be rapidly mobilized in the lungs to promote pathogen-specific immunity and to establish local mucosal immunity. In a mouse model of acute pneumonia with experimental strain 43,816 and clinical strain 7,29,088, immunization of HMVs via the lungs provided significant protection, reduced the number of colonies in deep tissues, improved survival, and reduced symptoms of acute inflammation. As expected, HMVs not only retained the immunogenicity of OMVs, but also effectively reduced the excessive immune response induced by OMVs. In conclusion, as a proof-of-concept for the design of antibacterial vaccines, the HMVs is an effective platform for activating bacteria-specific immunity, which can rapidly and effectively protect against nosocomial pneumonia caused by Klebsiella pneumoniae.
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