Preparation of chitosan/SiO2 coated silver nanoclusters and its application in enhanced fluorescence detection of berberine hydrochloride

Although the synthesis and applications of the metal nanoclusters and silica coated metal noclusters were widely explored, however, the fluorescence sensing application of silica coated metal nanoclusters are still challenges. In this work, the composite nanoparticles (PEI-Ag NCs/CSNPs) were synthesized by coating polyethyleneimine capped silver nanoclusters (PEI-Ag NCs) with chitosan/silica through a reverse microemulsion method, and then used for fluorescence enhancement detection of berberine hydrochloride (BRH). UV-vis absorption spectra, fluorescence spectra, IR spectra, transmission electron microscope (TEM) and X-ray Photoelectron Spectroscopy (XPS) technique were used to reveal the possible binding relationship between PEI-AgNCs and chitosan/silica and fluorescence sensing mechanism of PEI-Ag NCs/CSNPs to BRH. The results showed that PEI-Ag NCs/CSNPs possess better sensing ability as compared to the free PEI- Ag NCs and can be successfully applied to evaluate the BRH content in actual medicine.