立体选择性
化学
定向进化
级联
细胞色素
催化作用
细胞色素P450
立体化学
生物化学
酶
基因
突变体
色谱法
作者
Nan‐Wei Wan,Haibo Cui,Ling Zhao,Jing Shan,Ke Chen,Zhongqiang Wang,Xiaojian Zhou,Bao‐Dong Cui,Wen‐Yong Han,Yong‐Zheng Chen
摘要
A colorimetric high throughput screening method was developed based on a dual-enzyme cascade and used for the directed evolution of cytochrome P450 hydroxylase activity.
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