Strain engineering has played a key role in modern silicon electronics since 90 nm technology. Achieving similar advances within two-dimensional (2D) semiconductors is essential for their lab-to-fab transition. However, adapting silicon-based strain techniques to 2D transistors presents significant challenges; hence, previous studies are largely based on using a flexible substrate or nanocurved substrate, intrinsically limiting the practical application of 2D circuits. Here, we report a new strain engineering approach for 2D transistors without relying on the substrate, hence realizing strained 2D transistors on a standard flat and rigid SiO2 substrate. Importantly, the device strain could be directly visualized by the channel length change through a microscope rather than by previous indirect characterization methods, such as Raman or photoluminescence. Furthermore, an in situ electrical measurement is also conducted for the same MoS2 transistor under different strain values, and the monolayer carrier mobility increases linearly with the applied tensional strain, reaching an enhancement factor of 118%.