植入
骨愈合
牙科
骨质疏松症
医学
成骨细胞
碱性磷酸酶
H&E染色
骨细胞
血管内皮生长因子
染色
病理
化学
内科学
外科
血管内皮生长因子受体
体外
生物化学
酶
作者
Toetik Aryani,Aniek Setiya Budiatin,- Samirah,Aulia Maulidina,Aulia Intan Firdaus,Maria Apriliani Gani,Khoirotin Nisak,Junaidi Khotib,Alvi Jauharotus Syukriya
出处
期刊:Technology and Health Care
[IOS Press]
日期:2023-09-15
卷期号:31 (5): 1747-1757
被引量:1
摘要
BACKGROUND: Bone fracture is the main consequence of osteoporosis, which may become a neglected disease. OBJECTIVE: This study aims to fabricate bovine hydroxyapatite-gelatine (BHA-GEL) based bone-implant with alendronate (ALE) in vivo. METHODS: Wistar rats were used for an osteoporotic animal model induced by ovariectomy. There were three groups: negative control, BHA-GEL implant, and BHA-GEL-ALE implant. Each group performed a defect by drilling the femur (diameter of 2.2 mm and depth of 2 mm). Observations on the closure of bone defects were performed by X-ray radiography at the second and sixth week after surgery. The mechanism of bone healing was observed by using hematoxylin-eosin (HE) staining and immunohistochemical technique with anti-vascular endothelial growth factor (VEGF) and anti-alkaline phosphatase (ALP) antibodies. RESULTS: The radiograph examination showed the implanted group had accelerated bone growth. In addition, the osteoblast, osteoclast and osteocyte had accelerated migration to the defect area. Moreover, the immunoreactive score (IRS) of VEGF at the sixth week in the BHA-GEL-ALE group was lower than the other groups. Meanwhile, the IRS of ALP in BHA-GEL-ALE was higher compared to other groups. CONCLUSION: The BHA-GEL-ALE implant accelerates the healing of bone defect in the osteoporotic rat by increasing the ALP expression and the total number of cells.
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