病毒学
中和
抗体
牛痘
免疫系统
鼻腔给药
中和抗体
免疫
病毒
重组DNA
生物
免疫学
痘病毒科
作者
Xiaoling Cao,Junjie Zai,Qingzhen Zhao,Lilan Xie,Yaoming Li
出处
期刊:Vaccine
[Elsevier]
日期:2022-08-01
标识
DOI:10.1016/j.vaccine.2022.08.054
摘要
Respiratory transmission of SARS-CoV-2 is considered to be the major dissemination route for COVID-19, therefore, mucosal immune responses have great importance in preventing SARS-CoV-2 from infection. In this study, we constructed a recombinant Vaccinia virus (VV) harboring trimeric receptor-binding domain (RBD) of SARS-CoV-2 spike protein (VV -tRBD ), and evaluated the immune responses towards RBD following intranasal immunization against mice and rabbits. In BALB/c mice, intranasal immunization with VV -tRBD elicited robust humoral and cellular immune responses, with high-level of both neutralizing IgG and IgA in sera against SARS-CoV-2 psudoviruses, and a number of RBD-specific IFN-γ-secreting lymphocytes. Sera from immunized rabbits also exhibited neutralization effects. Notably, RBD-specific secretory IgA (sIgA) in both nasal washes and bronchoalveolar lavage fluids (BALs) were detectable and showed substantial neutralization activities. Collectively, a recombinant VV expressing trimeric RBD confers robust systemic immune response and mucosal neutralizing antibodies, thus warranting further exploration as a mucosal vaccine.
科研通智能强力驱动
Strongly Powered by AbleSci AI