狗尾草
狐尾
MYB公司
农学
生物
转录因子
转基因水稻
氮气
狗尾草
植物
转基因作物
转基因
基因
遗传学
化学
有机化学
杂草
作者
Yuewei Zhang,Zhang He,Xin Qi,Maomao Li,Jin Liu,Si Quang Le,Kai Chen,Chunxiao Wang,Yongbin Zhou,Zhao‐Shi Xu,Jun Chen,Changhong Guo,Wensi Tang,You‐Zhi Ma,Ming Chen
标识
DOI:10.1016/j.plaphy.2023.02.025
摘要
Nitrogen fertilizers significantly increase crop yield; however, the negative impact of excessive nitrogen use on the environment and soil requires urgent attention. Improving crop nitrogen use efficiency (NUE) is crucial to increase yields and protect the environment. Foxtail millet (Setaria italica L.), a gramineous crop with significant tolerance to barren croplands, is an ideal model crop for studying abiotic stress resistance in gramineous crops. However, knowledge of the regulatory network for NUE in foxtail millet is fragmentary. Herein, we identified an R2R3-like MYB transcription factor in foxtail millet, SiMYB30, which belongs to MYB subfamily 17. The expression of SiMYB30 is responsive to low nitrogen (LN) concentration. Compared with wildtype Kitaake, seedlings of rice lines overexpressing SiMYB30 showed significantly increased shoot fresh and dry weights, plant height, and root area under LN treatment indoors. Consistently, overexpression of SiMYB30 in field experiments significantly increased grain and stem nitrogen contents, grain yield per plant, and stem weight in rice. Furthermore, qRT-PCR revealed that SiMYB30 effectively activated the expression of nitrogen uptake-related genes—OsNRT1, OsNRT1.1B, and OsNPF2.4—and nitrogen assimilation-related genes—OsGOGAT1, OsGOGAT2, and OsNIA2. Notably, SiMYB30 directly bound to the promoter of OsGOGAT2 and regulated its expression. These results highlight the novel and pivotal role of SiMYB30 in improving crop NUE.
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