Growth and Manipulation of Yeast

Abstract Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures. This unit describes culture conditions for these basic techniques. Additional methods describe determination of yeast mating type, diploid construction, sporulation, tetrad dissection, and random spore analysis. Curr. Protoc. Mol. Biol . 82:13.2.1‐13.2.12. © 2008 by John Wiley & Sons, Inc.