生物
诱导多能干细胞
细胞生物学
Wnt信号通路
干细胞
胚胎干细胞
外胚层
成纤维细胞生长因子
胚芽层
细胞分化
遗传学
细胞效价
信号转导
基因
原肠化
受体
作者
Lei Yu,Yulei Wei,Hai Xi Sun,Ahmed K. Mahdi,Carlos A. Pinzón-Arteaga,Masahiro Sakurai,Daniel A. Schmitz,Canbin Zheng,Emily Ballard,Jie Li,Noriko Tanaka,Aoi Kohara,Daiji Okamura,A. Mutto,Ying Gu,Pablo J. Ross,Jun Wu
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2021-03-01
卷期号:28 (3): 550-567.e12
被引量:117
标识
DOI:10.1016/j.stem.2020.11.003
摘要
Summary
Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. To date, however, no known PSCs have demonstrated dual competency for chimera formation and direct responsiveness to primordial germ cell (PGC) specification, a unique functional feature of formative pluripotency. Here, by modulating fibroblast growth factor (FGF), transforming growth factor β (TGF-β), and WNT pathways, we derived PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and are capable of contributing to intra- or inter-species chimeras in vivo. XPSCs represent a pluripotency state between naive and primed pluripotency and harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency. XPSCs open new avenues for studying mammalian pluripotency and dissecting the molecular mechanisms governing PGC specification. Our method may be broadly applicable for the derivation of analogous stem cells from other mammalian species.
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