脚手架
材料科学
组织工程
生物医学工程
明胶
静电纺丝
纳米纤维
PLGA公司
再生(生物学)
血管
生物物理学
纳米技术
化学
聚合物
复合材料
细胞生物学
医学
心理学
生物化学
精神科
纳米颗粒
生物
作者
Xingmao Li,Lin Huang,Long Li,Ya Hui Tang,Qibin Liu,Haibo Xie,Jialiang Tian,Shaobing Zhou,Geng Tang
标识
DOI:10.1080/09205063.2019.1697171
摘要
Natural blood vessels have a multi-layered, cell-specific oriented spatial structure, mimicking of this structure is a promising way for blood vessel regeneration. In this study, a newly developed dual-oriented/bilayered small-diameter tubular scaffold was electrospun using a mixture of poly (ε-caprolactone) (PCL), poly (D, L-lactide-co-glycolide) (PLGA) and gelatin. The nanofiber orientations of the bilayers were spatially perpendicular to each other, aiming at guiding cell-specific orientation of smooth muscle cells (SMCs) and endothelial cells (ECs) in vitro respectively. The results showed that the hydrophilicity of scaffold was greatly improved by gelatin, and the mechanical property of this scaffold was the best among all. The in vitro degradation demonstrated that by mixing of three biodegradable polymers, a relatively fast degradation rate was achieved. After SMCs and ECs were seeded on scaffolds, cell viability, cellular morphology, and cytoskeleton behavior were investigated. The results revealed that as-electrospun scaffolds could promote both SMCs and ECs proliferation. Moreover, topographic cues offered by oriented nanofibers could guide the growth and orientation of SMCs and ECs. Therefore, the dual-oriented/bilayered electrospun scaffold is a superior structural and functional analogue to natural blood vessel and a potential candidate for vascular tissue engineering.
科研通智能强力驱动
Strongly Powered by AbleSci AI