A novel fluorescent probe for H2O2 detection based on CdSe@ZnS quantum dots/Ag nanocluster hybrid

The selective and quantitative detection of H2O2 is important for its employment in physiological, environmental and industrial applications. In this paper, a sensitive and selective strategy for H2O2 detection was established based on the fluorescence quenching of [email protected] quantum dots (QDs) by H2O2-mediated etching process of Ag nanoclusters (AgNCs). In this strategy, dihydrolipoic acid (DHLA) modified AgNCs were applied as H2O2 response group, the existence of H2O2 could initiate the oxidation of AgNCs and the production of Ag+. [email protected] QDs are very sensitive to Ag+, which could give rise to the effective fluorescence quenching of [email protected] QDs. Based on this strategy, the present fluorescent assay could realize the quantification detection of H2O2 and the limit of detection is calculated to be 0.3 μM under the optimum conditions. Furthermore, [email protected]/AgNCs hybrid-based probe was applied to detecting H2O2 in milk samples and showed a good recoveries results ranged from 95.8% to 112.0%, meaning the potential applicability of this strategy.