Effect of cisplatin on ototoxicity and expression of caspase-3 in mouse cochlea

Objective To establish a mice model of cisplatin-induced ototoxicity and to investigate the effect of cisplatin on the expression of caspase-3 in mouse cochlea.Methods Totally 69 Kunming mice were randomly divided into control group,cisplatin 2.5mg/(kg·d) group,cisplatin 3.5mg/(kg·d) group and cisplatin 4.5mg/(kg·d)group.Mice were injected intraperitoneally for 5 days.Auditory brainstem response(ABR) was measured to observe the change of hearing.Envision method of immunohistochemistry was applied to detect the expression of caspase-3 in cochlea.Results The weight and hearing of mice in different dose cisplatin groups were declined significantly as compared with those in control group(P0.05,P0.01),and the expression of caspase-3 was greater remarkably with more cisplatin injected.Conclusion A mouse model of cisplatin-induced ototoxicity could be established.Caspase-3 is involved in cisplatin-induced cochlear injury.It suggests that apoptosis may be one of the mechanisms of cisplatin ototoxicity.