Ply twist required to produce balanced two-ply cotton rotor yarn has been measured by placing the two-ply yarn freely in water. When the two-ply yarns are placed freely in water, the ply twist remaining in the yarn is about 40-45 % of single yarn twist, irrespective of ply-to-single yarn twist ratio used for the production of yarn. The twist liveliness of two-ply yarns both in dry and wet states increases as the ply-to-single yarn twist ratio increases. The ply yarn with 45% of single yarn twist shows the lowest amount of snarling twist both in dry and wet states. The fabrics produced using the two-ply yarn with 45% of single yarn twist show negligible amount of spirality in both dry and wet relaxed conditions.