Optimization on Extraction of Total Flavonoids from Glechoma longituba with Ultrasonic Wave by Central Composite Design-Response Surface Method

OBJECTIVE To study the optimum condition of extraction for the total flavonoids in Glechoma longituba by central composite design-response surface method.METHODS Independent variables were methanol concentration,ultrasonic time and solvent,while dependent variables were the content of total flavonoids.Central composite design-response surface method(CCD-RSM) were used to optimize the extracting process.RESULTS The content of extraction conditions was 55% methanol,ultrasound 90 min and 8 times the amount of solvent.CONCLUSION CCD-RSM can be used to optimize extraction of total flavonoids of Glechoma longituba,and the optimum mathematic model is highly predictive and the experimental design methods have higher reliability.