Objective:To determine the negative rates of pathogens DNA in male sexually transmitted urethritis(STU) patients, after treatment, with sparfloxacin(200 mg, once a day, for 7 days)and sparfloxacin (200 mg,once a day, for 7 days)allied azithromycin(1 g,once a day, for 3 days). Methods:We selected the male patients with STU for the first time as subjects, and then divided them into two group randomly. The control group was treated with sparfloxacin, the experiment group was treated with sparfloxacin and azithromycin. Results:After treatment, the negative rates of pathogens DNA are 47.3% and 66.7%, respectively, in the control group and the experiment group. Conclusion:The negative-rates of pathogens DNA are low in both groups, but combined use of sparfloxacin and azithromgcin yield a higher negative rate than that of sparfloxacin.