Shared and distinct functions of two G ti1/ P ac2 family proteins in growth, morphogenesis and pathogenicity of M agnaporthe oryzae

Summary G ti1/ P ac2 are conserved family proteins that regulate morphogenic transition in yeasts such as S chizosaccharomyces pombe and C andida albicans , and they also control toxin production and pathogenicity in filamentous fungus F usarium graminearum . To test the functions of G ti1/ P ac2 paralogues MoGti1 and MoPac2 in the rice blast fungus M agnaporthe oryzae , we generated respective Δ M ogti1 and Δ M opac2 mutant strains. We found that MoGti1 and MoPac2 exhibit shared and distinct roles in hyphal growth, conidiation, sexual reproduction, stress responses, surface hydrophobility, invasive hyphal growth and pathogenicity. Consistent with the putative conserved function of MoGti1 , we showed that MoGti1 ‐ GFP is localized to the nucleus, whereas MoPac2 ‐ GFP is mainly found in the cytoplasm. In addition, we provided evidence that the nuclear localization of MoGti1 could be subject to regulation by MoPmk1 mitogen‐activated protein kinase. Moreover, we found that the reduced pathogenicity in the Δ M opac2 mutant corresponds with an increased expression of plant defence genes, including PR1a , AOS2 , LOX1 , PAD4 , and CHT1 . Taken together, our studies provide a comprehensive analysis of two similar but distinct G ti1/ P ac2 family proteins in M . oryzae , which underlines the important yet conserved functions of these family proteins in plant pathogenic fungi.