Synthetic Biology Toolbox and Chassis Development in Bacillus subtilis

Recent development of synthetic biology toolboxes for B. subtilis, including gene expression regulatory toolboxes and genome-wide editing tools, provides powerful tools for precise gene expression control and efficient genome editing. Advances of B. subtilis chassis and their applications help to understand fundamental cellular processes and techniques for improving production of biomolecules or heterologous enzymes. Comparing B. subtilis chassis development with E. coli and S. cerevisiae chassis may provide potential directions for B. subtilis chassis construction. Based on technical advances in the sequencing and synthesis of genetic components as well as the genome, significant progress has recently been made in developing synthetic biology toolboxes and chassis for the model Gram-positive bacterium Bacillus subtilis. In this review, we discuss recently developed synthetic biology toolboxes, including gene expression toolsets and genome editing tools. Next, advances in the B. subtilis chassis and its applications are discussed in comparison to those of other model microorganisms. Finally, future directions for the integrative use of B. subtilis synthetic biology tools and the development of an advanced chassis for efficient biomanufacturing are discussed. These factors are expected to become a major driving force for facilitating biotechnological applications of B. subtilis. Based on technical advances in the sequencing and synthesis of genetic components as well as the genome, significant progress has recently been made in developing synthetic biology toolboxes and chassis for the model Gram-positive bacterium Bacillus subtilis. In this review, we discuss recently developed synthetic biology toolboxes, including gene expression toolsets and genome editing tools. Next, advances in the B. subtilis chassis and its applications are discussed in comparison to those of other model microorganisms. Finally, future directions for the integrative use of B. subtilis synthetic biology tools and the development of an advanced chassis for efficient biomanufacturing are discussed. These factors are expected to become a major driving force for facilitating biotechnological applications of B. subtilis. modulation of abundance or activities of key pathway enzymes to obtain optimal and balanced relative activities of pathway enzymes in metabolic pathways for improve metabolic flux. a strategy for obtaining an engineered host with reduced genome size by large-scale deletion; for example, to understand the fundamental cellular processes of living cells and construct a cell factory to improve the production of enzymes or biomolecules. engineered hosts for enzyme or biochemical production based on synthetic biology strategies. Synthetic pathways and synthetic devices can be introduced into the synthetic biology chassis cell to test feasibility and efficacy of designed synthetic pathways and synthetic devices. consists of genetic regulatory elements and genetic engineering approaches, which provides a basis for constructing synthetic pathways, genetic circuits and synthetic biology chassis.