脂肪酶
介孔材料
固定化酶
化学工程
碳纳米管
介孔二氧化硅
生物传感器
材料科学
三丁酸甘油酯
化学
电极
比表面积
催化作用
纳米技术
有机化学
酶
工程类
物理化学
作者
Ashok Kumar,Gi Dae Park,Sanjay Patel,Sanath Kondaveeti,Sachin V. Otari,Muhammad Zahid Anwar,Vipin Chandra Kalia,Yogendra Singh,Sun Chang Kim,Byung-Kwan Cho,Jae Hak Sohn,Dong Rip Kim,Yun Chan Kang,Jung-Kul Lee
标识
DOI:10.1016/j.cej.2018.11.052
摘要
Novel mesoporous SiO2 microparticles were synthesized by spray pyrolysis using multiwalled carbon nanotubes (MCNTs) as a template. The synthesized multicompartment structure with uniform pores of 12.0 nm was used to immobilize lipase from Thermomyces lanuginosus. The total surface area of mesoporous SiO2 microparticles prepared from silica colloidal solution was increased by 26-folds compared to that of dense SiO2 particles (494 vs 19.0 m2 g−1, respectively). Mesoporous SiO2 particles showed 236% higher protein loading for lipase, than dense SiO2 particles. The maximum velocity (Vmax) and catalytic efficiencies of immobilized lipase were 3.80 and 5.90 folds higher than that of free enzyme. Contact angle analysis revealed increased hydrophobicity of the mesoporous particles, which is advantageous for lid opening at the active center, and increased activity after immobilization. We next developed a lipase/SiO2/glassy carbon electrode (GCE) biosensors. Cyclic voltammetric results showed linear responses of the lipase/SiO2/GCE bioelectrode towards tributyrin (50–300 mg dL−1) as a surface-limited reaction in Tris-HCl buffer. After 12 repetitive uses, dense SiO2- and mesoporous SiO2-bound lipase retained 74.2 and 95.4% of its original activities, respectively. Thus, given their desirable characteristics and industrial utility, greatly porous SiO2 particles may provide an excellent support for enzyme immobilization in biosensor development or biocatalysis in organic media.
科研通智能强力驱动
Strongly Powered by AbleSci AI