管道(软件)
吞吐量
计算机科学
计算生物学
高通量筛选
软件
管道运输
作者
Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Kumar Thakor,Simon Kling,Tomas Kalina,Tuula A. Nyman,Maria Stensland,Wei Zhou,Gustavo Antonio De Souza,Lars Holden,Jan Stuchly,Markus F. Templin,Fridtjof Lund-Johansen
出处
期刊:Nature Methods
[Springer Nature]
日期:2018-10-30
卷期号:15 (11): 909-912
被引量:20
标识
DOI:10.1038/s41592-018-0179-8
摘要
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
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