脂肪生成
间充质干细胞
Wnt信号通路
细胞生物学
化学
小RNA
骨髓
油红O
信号转导
生物
免疫学
生物化学
基因
作者
Siyan Yi,Rui Yang,Rui Mu,Yang Yu,Rong Liang,Lei Jin
出处
期刊:Life Sciences
[Elsevier]
日期:2019-03-01
卷期号:220: 84-91
被引量:27
标识
DOI:10.1016/j.lfs.2019.01.051
摘要
A number of evidences suggest that microRNAs are involved in the adipogenic commitment of mesenchymal stem cells (MSCs). Recent studies have investigated that miR-199a-3p played a pivotal role in adipocyte differentiation. However, the detailed mechanism in this complex biological process remains largely unknown. In current study, we found that the expression of miR-199a-3p was gradually increased during adipogenic differentiation of bone marrow derived mesenchymal stem cells (BMMSCs). Enhanced expression of miR-199a-3p promoted adipogenesis, whereas silence of miR-199a-3p rescued BMMSCs from adipogenic commitment. For further mechanism exploration, KDM6A was confirmed to be the target of miR-199a-3p and the expression of KDM6A was gradually decreased during adipogenic differentiation of BMMSCs. Furthermore, up-regulation of KDM6A markedly abolished the miR-199a-3p overexpression induced adipogenic augmentation, whereas down-regulation of KDM6A suppressed the adipogenic reduction caused by miR-199a-3p silence. In addition, WNT signaling was also verified to be the downstream of miR-199a-3p/KDM6A to regulate adipogenic differentiation of BMMSCs. Taken together, current results indicate that miR-199a-3p regulate adipogenesis of BMMSCs by targeting KDM6A/WNT signaling, which highlights a new insight for a better understanding of molecular mechanism and stem cell based therapy on osteoporotic diseases.
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