Response of methanogenic granules enhanced by magnetite to ammonia stress

Excessive ammonia has an inhibitory effect on anaerobic granular sludge (AnGS) when treating industrial wastewater with high concentration of ammonia and organic matters. The addition of conductive materials has been widely reported to improve the AnGS activity, which has the potential to alleviate the ammonia inhibition. In this study, the addition of magnetite was carried out to enhance the activity of AnGS in UASB reactor, then the response of AnGS to different ammonia levels was investigated. Results showed that magnetite facilitated the enrichment of Methanosaeta and Clostridium sensu stricto 1. Under the ammonia stress (up to 5 g TAN/L), it was interesting that Methanosaeta was better retained (abundance of 45.8%), and the abundance of ammonia-resistant Clostridium sensu stricto 1 increased to 34.3% in presence of magnetite. RT-qPCR analysis revealed that Methanosaeta could maintain metabolically active for counteracting the ammonia inhibition along with the higher transcription of genes encoding for CO2-dependent pathway. The electron transport activity and ATP content of AnGS were 1.25-2.12 and 1.23-2.56 folds higher than those in the control group, respectively. In addition, the AnGS could maintain the stability of structure because Methanosaeta was the skeleton of AnGS. As a result, the analysis of enzyme activity showed that the overall methanogenic metabolism was more active, thus ensured the effective operation of UASB reactor. This study provided the scientific understanding about the role of magnetite to alleviate the ammonia inhibition, and had important implications for stable treatment and recycling of industrial wastewater.