光动力疗法
流式细胞术
免疫原性细胞死亡
免疫系统
细胞凋亡
CD8型
癌症研究
光敏剂
体内
程序性细胞死亡
分子生物学
化学
生物
免疫疗法
免疫学
有机化学
生物技术
生物化学
作者
Shan Long,Yibing Zhao,Yanyan Xu,Hui Li,He Zhao,Defu Chen,Jing Zeng,Haixia Qiu,Xiaosong Li,Yajia Gu
标识
DOI:10.1142/s1793545822400028
摘要
Photodynamic therapy (PDT) not only destroys tumor cells directly but also induced anti-tumor immune response through damage-associated molecular patterns (DAMPs). It is reported that anti-tumor response was associated with light dose and photosensitizer used in PDT. In this study, 4T1 tumor cells were implanted on both the right and left flanks of mice. Only the right tumor was treated by HpD-PDT, while the left tumor was not irradiated. The anti-tumor immune response induced by HpD-PDT was investigated. The expression of DAMPs and co-stimulatory molecules induced by HpD-PDT were tested by immunofluorescence and flow cytometry in vivo. Different light doses of PDT were designed to treat 4T1 cells. The killing effect was assessed by CCK-8 kit and apoptosis kit. The expression of DAMPs on 4T1 cells after HpD-PDT were evaluated by flow cytometry, western blot and ATP kit. This study showed that CD4[Formula: see text]T, CD8[Formula: see text]T and the production of IFN-[Formula: see text] were increased significantly on day 10 in right-tumor after PDT treatment compared with control group. HpD-PDT enhanced the expression of calreticulin (CRT) on tumor tissue. Importantly, co-stimulatory molecular OX-40 and 4-1BB were elevated on CD8[Formula: see text]T cells. In vitro, immunogenic death of 4T1 cells was induced after PDT. Besides, the expression of DAMPs increased with the increasing of energy density. This study indicates that anti-tumor immune effect was induced by HpD-PDT. The knowledge of the involvement of CRT, ATP and co-stimulatory molecules uncovers important mechanistic insight into the anti-tumor immunogenicity. It was the first time that co-stimulatory molecules were investigated and found to elevate after PDT.
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