Improving the robustness of a catalyzed hairpin assembly with a three-arm nanostructure for nonenzymatic signal amplification

A nonenzymatic and isothermal signal amplification was performed by a 3-arm structure based on a catalyzed hairpin DNA assembly (3-CHA). By suppressing the leakage pathway, the sensitivity (<30 pM LOD) and selectivity of the 3-CHA were improved.