Genetic and biological properties of H10N3 avian influenza viruses: A potential pandemic candidate?

Transboundary and Emerging DiseasesEarly View ORIGINAL ARTICLE Genetic and biological properties of H10N3 avian influenza viruses: A potential pandemic candidate? Yanna Guo, Yanna Guo 836567594@qq.com orcid.org/0000-0003-4561-5378 MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorPingyun Ding, Pingyun Ding 495239552@qq.com College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorYinjing Li, Yinjing Li 1052958440@qq.com MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorYaping Zhang, Yaping Zhang zyp412321@163.com State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute in CAAS, Harbin, ChinaSearch for more papers by this authorYiqing Zheng, Yiqing Zheng zyiqing405@163.com MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorMengqi Yu, Mengqi Yu 782187507@qq.com MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorYasuo Suzuki, Yasuo Suzuki suzukiy@isc.chubu.ac.jp College of Life and Health Sciences, Chubu University, Aichi, JapanSearch for more papers by this authorHaitao Zhang, Haitao Zhang HT_z1@126.com Lihua Nanjing Industrial Research Institute Co., Ltd, Nanjing, ChinaSearch for more papers by this authorJihui Ping, Corresponding Author Jihui Ping jihui.ping@njau.edu.cn MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China Correspondence Jihui Ping, MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Email: jihui.ping@njau.edu.cnSearch for more papers by this author Yanna Guo, Yanna Guo 836567594@qq.com orcid.org/0000-0003-4561-5378 MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorPingyun Ding, Pingyun Ding 495239552@qq.com College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaSearch for more papers by this authorYinjing Li, Yinjing Li 1052958440@qq.com MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorYaping Zhang, Yaping Zhang zyp412321@163.com State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute in CAAS, Harbin, ChinaSearch for more papers by this authorYiqing Zheng, Yiqing Zheng zyiqing405@163.com MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorMengqi Yu, Mengqi Yu 782187507@qq.com MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaSearch for more papers by this authorYasuo Suzuki, Yasuo Suzuki suzukiy@isc.chubu.ac.jp College of Life and Health Sciences, Chubu University, Aichi, JapanSearch for more papers by this authorHaitao Zhang, Haitao Zhang HT_z1@126.com Lihua Nanjing Industrial Research Institute Co., Ltd, Nanjing, ChinaSearch for more papers by this authorJihui Ping, Corresponding Author Jihui Ping jihui.ping@njau.edu.cn MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China Correspondence Jihui Ping, MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Email: jihui.ping@njau.edu.cnSearch for more papers by this author First published: 23 January 2022 https://doi.org/10.1111/tbed.14458Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The continued emergence of human illness caused by avian influenza viruses (AIVs) demonstrates the threat of strains such as H5N1, H7N9, H10N8, and now H10N3. The genetic and biological properties of H10N3 viruses are not fully understood. In this study, three H10N3 strains isolated from live poultry markets (LPMs) were systematically studied. Genome sequencing showed that the poultry-origin viruses are highly homologous to the human H10N3 isolate. The three avian strains were A/chicken/Jiangsu/0146/2021(abbreviated as JS146, H10N3), A/chicken/Jiangsu/0169/2021 (JS169, H10N3), and A/chicken/Jiangsu/0189/2021(JS189, H10N3). Animal studies indicated that all three viruses are highly pathogenic to mice and that all could replicate efficiently in mouse nasal turbinate and lungs despite maintaining their avian receptor binding affinity. We also found that these viruses replicated efficiently in A549 cells and chicken embryos. The strain JS146 had sensitivity to the neuraminidase-targeting drugs oseltamivir and zanamivir, whereas JS169 and JS189 were more resistant; genetic comparison implied that a substitution at NA position 368 conferred drug resistance. Importantly, several key molecular markers associated with mammalian adaptation had been detected in both avian and human-isolated H10N3 influenza viruses in the HA (G228S), PB2 (I292V and A588V), PB1 (M317V and I368V), and PA (A343S, K356R and S409N) protein. The above work contributes new insight into the biology of this potentially zoonotic subtype and provides evidence supporting the continued epidemiological monitoring of human infections caused by AIV subtype H10N3. Open Research DATA AVAILABILITY STATEMENT The nucleotide sequences of the three avian-origin H10N3 viruses A/chicken/Jiangsu/0146/2021(H10N3), A/chicken/Jiangsu/0169/2021(H10N3), and A/chicken/Jiangsu/0189/2021(H10N3) have been deposited in GenBank under accession numbers (OL636797-OL636804), (OL638114-OL638121), and (OL638123-OL638130), respectively. 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