嘌呤霉素
类囊体
膜
叶绿体
核糖体
生物化学
生物物理学
衣原体
化学
生物
蛋白质生物合成
核糖核酸
基因
突变体
作者
Maurice M. Margulies,H. Lee Tiffany,Allan Michaels
标识
DOI:10.1016/0006-291x(75)90381-2
摘要
Chloroplast thylakoids with attached ribosomes were isolated from Chlamydomonas reinhardti. They were allowed to incorporate labeled amino acids into polypeptides. Labeled membranes were recovered from the reaction mixture, and a portion was treated with puromycin. The amount of labeled polypeptides released to the medium, and to the membranes by puromycin was determined by comparing radioactivity in soluble protein before, and after untreated, and puromycin-treated membranes were solubilized with the detergent Nonidet P-40. About 20% of the radioactive protein associated with the membranes was in nascent chains which were terminated by puromycin. Essentially all of terminated nascent chains remained with the membranes, and thus, were vectorially released. The results support the hypothesis that polypeptides which are synthesized by thylakoid-bound ribosomes are being incorporated into the membranes as they are synthesized.
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