Formation and Stability of a Janus-Wedge Type of DNA Triplex

A new type of DNA targeting with the formation of a Janus-Wedge (J-W) triple helix is described. The "wedge" residue (W) attached to a PNA backbone is designed to insert itself into double-stranded DNA and base pair with both Watson−Crick faces. To study the stability of such an assembly, we have examined the formation of the J-W triplex with dC8 − T8 target sequence. The use of this target sequence permits the study of this new helix form without competing Watson−Crick interactions between the two target residues. Studies indicate that the W strand binds to both target strands, with defined polarity and a stability (−15.2 kcal/mol) that is roughly the sum of the two independent duplex interactions.