活性氧
过氧化氢
联苯胺
单线态氧
体内
超氧化物
化学
细胞生物学
氧气
氧化应激
生物化学
信号通路
生物物理学
生物
信号转导
酶
遗传学
有机化学
作者
Steven M. Driever,Michael J. Fryer,Philip M. Mullineaux,Neil R. Baker
出处
期刊:Methods in molecular biology
日期:2009-01-01
卷期号:: 109-116
被引量:46
标识
DOI:10.1007/978-1-59745-289-2_7
摘要
Reactive oxygen species (ROS) are involved in many signalling pathways and numerous stress responses in plants. Consequently, it is important to be able to identify and localize ROS in vivo to evaluate their roles in signalling. A number of probes that have a high affinity for specific ROS and that are effectively taken up by cells and tissues are commercially available. Applications to intact leaves of singlet oxygen sensor green (SOSG), nitroblue tetrazolium (NBT), di-amino benzidine (DAB) and Amplex Red to detect singlet oxygen, superoxide and hydrogen peroxide are described. Imaging of the probes in the cells and tissues of leaves allows sites of ROS production to be identified.
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