PI3K/AKT/mTOR通路
活力测定
次氯酸钠
化学
碱性磷酸酶
牙周膜干细胞
运行x2
细胞生物学
次氯酸盐
鱼腥草素骨
自噬
MTT法
蛋白激酶B
牙髓干细胞
干细胞
骨钙素
分子生物学
细胞生长
生物
信号转导
生物化学
细胞
细胞凋亡
酶
有机化学
作者
Mohamed M. Elashiry,Shereen N. Raafat,Franklin R. Tay,Shehabeldin Mohamed Saber
出处
期刊:Life Sciences
[Elsevier]
日期:2023-09-01
卷期号:329: 121989-121989
标识
DOI:10.1016/j.lfs.2023.121989
摘要
The present study investigated the effect of rapamycin on the viability and osteogenic differentiation potential of human periodontal ligament stem cells (hPDLSCs) in the presence of sodium hypochlorite (NaOCl).After determining the minimum inhibitory concentration of NaOCl and optimum concentration of rapamycin, the viability of hPDLSCs was evaluated using the MTT assay subsequent to their exposure to NaOCl, rapamycin, or a combination of both. Osteogenic differentiation was evaluated by the cell mineralization assay performed by alizarin red S staining, alkaline phosphatase activity, and monitoring the expression of osteogenic genes markers Runt-related transcription factor 2, osteocalcin, and osteoprotegerin, using real-time quantitative polymerase chain reaction (RT-qPCR). The expression of autophagy-related genes PI3K, Akt, and mTOR, was also analyzed with RT-qPCR.Stem cells treated with rapamycin showed the highest percentage of viable cells in the presence of NaOCl. The same trend was observed for all osteogenic differentiation assays. The hPDLSCs treated with rapamycin demonstrated the highest calcium nodule deposition, alkaline phosphatase activity, and the expression of osteogenic gene markers. These effects were not adversely affected by the presence of NaOCl. Rapamycin significantly inhibited mTOR gene expression, while there were no differences in the gene expression of PI3K and Akt.Rapamycin counteracts the cytotoxic effect of NaOCl by enhancing the viability and osteogenic differentiation potential of hPDLSCs. Rapamycin appears to accomplish these processes via autophagy activation, by inhibiting mTOR gene expression. The incorporation of rapamycin in regenerative endodontic therapy may encourage a higher success rate.
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