High-yield and scalable cellulose nanomesh preparation via dilute acid vapor and enzymatic hydrolysis-mediated nanofabrication

Nanocellulose has received considerable attention in diverse research fields owing to its unique nanostructure-mediated physicochemical properties. However, classical acid hydrolysis usually destroys the microstructural integrity of cellulose, leading to the violent dissociation of cellulose into low-dimensional nanofibers and limiting the formation of intact structures with high specific surface areas. Herein, we have optimized the methodology of dilute acid vapor hydrolysis combined with the enzymatic hydrolysis (DAVE) method and investigated the pore formation mechanism of cellulose nanomesh (CNM). Benefiting from the selective nano-engraving effect of hydrochloric acid vapor on the amorphous region of cellulose followed by widening of the three-dimensional nanopores using enzymatic hydrolysis, confirmed by topographic, spectroscopic, and crystallographic tests, the as-prepared CNM, significantly different from the existing nanocellulose, exhibited improved specific surface area (98.37 m2/g), high yield (88.5 %), high crystallinity (73.4 %), and excellent thermal stability (375.4 °C). The proposed DAVE approach may open a new avenue for nanocellulose manufacturing.