抗细菌
支原体
枯草芽孢杆菌
微生物学
生物
铁载体
分枝杆菌
抗菌剂
内生菌
最小抑制浓度
乙酸乙酯
结核分枝杆菌
细菌
生物化学
肺结核
植物
医学
病理
遗传学
作者
Yedukondalu Nalli,Sanju Singh,Apexa Gajjar,B. Mahizhaveni,V.N. Azger Dusthackeer,Pramod B. Shinde
出处
期刊:PubMed
日期:2023-02-16
卷期号:76 (2)
被引量:6
标识
DOI:10.1093/lambio/ovac026
摘要
The bacterial endophytes isolated from the halophyte Salicornia brachiata were explored for the antimicrobial potential to discover novel microbial inhibitors that combat multidrug resistance. Upon investigation, ethyl acetate extract of the endophyte Bacillus subtilis NPROOT3 displayed significant potency against Mycobacterium smegmatis MTCC6 as well as Mycobacterium tuberculosis H37Rv strain. Further investigation of ethyl acetate crude extract by repeated chromatographic separations followed by characterization using UV, HR-ESI-MS, MALDI-MS, MALDI-MS/MS, CD, and NMR spectroscopy yielded a series of five known siderophores, namely, SVK21 (1), bacillibactin C (2), bacillibactin B (3), tribenglthin A (4), and bacillibactin (5). A total of two out of five compounds, 4 (MIC 38.66 μM) and 5 (MIC 22.15 μM) exhibited significant inhibition against the strain M. smegmatis MTCC6 comparable with positive control rifampicin (MIC 12.15 μM). None of these five bacillibactin molecules are previously reported to exhibit bioactivity against Mycobacterium sp. Herein for the first time, all the compounds were screened for their antibacterial activities against a panel of bacterial pathogens of humans. Furthermore, the probable mechanism of action of bacillibactin compounds for their antimycobacterial activity is also discussed. The findings of this study open up a new chemotype for inhibition of the Mycobacterium sp. and other multidrug-resistant pathogens.
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