微流控
数字微流体
炸薯条
基质(化学分析)
微流控芯片
化学
计算机科学
材料科学
纳米技术
色谱法
光电子学
电信
电润湿
电介质
作者
Zhicheng Yang,Kai Jin,Yimin Chen,Liu Qian,Hongxu Chen,Siyi Hu,Yuqiu Wang,Zilu Pan,Fang Fěng,Mingmin Shi,Hua Xie,Hanbin Ma,Hu Zhou
出处
期刊:JACS Au
[American Chemical Society]
日期:2024-03-26
标识
DOI:10.1021/jacsau.4c00027
摘要
Single-cell proteomics offers unparalleled insights into cellular diversity and molecular mechanisms, enabling a deeper understanding of complex biological processes at the individual cell level. Here, we develop an integrated sample processing on an active-matrix digital microfluidic chip for single-cell proteomics (AM-DMF-SCP). Employing the AM-DMF-SCP approach and data-independent acquisition (DIA), we identify an average of 2258 protein groups in single HeLa cells within 15 min of the liquid chromatography gradient. We performed comparative analyses of three tumor cell lines: HeLa, A549, and HepG2, and machine learning was utilized to identify the unique features of these cell lines. Applying the AM-DMF-SCP to characterize the proteomes of a third-generation EGFR inhibitor, ASK120067-resistant cells (67R) and their parental NCI-H1975 cells, we observed a potential correlation between elevated VIM expression and 67R resistance, which is consistent with the findings from bulk sample analyses. These results suggest that AM-DMF-SCP is an automated, robust, and sensitive platform for single-cell proteomics and demonstrate the potential for providing valuable insights into cellular mechanisms.
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