High biocompatible nitrogen and sulfur Co-doped carbon dots for Hg(II) detection and their long-term biological stability in living cells

Fluorescent carbon dots have been highly reported nanomaterials in recent times because of their excellent physio-chemical properties and various field of applications. Herein, a one-step hydrothermal approach was used to synthesize high biocompatible nitrogen and sulfur co-doped carbon dots, and examined their chemical sensing (Hg2+) and biological imaging properties. The N,S-CDs exhibited blue light, demonstrating a high quantum yield of up to 44.5% and excitation-independent fluorescent characteristics. Cytotoxicity was observed by CCK-8 assay using T-ca cells as a target source. Cell viability was recorded over 80% even after 7 days of treatment with a concentration up to 400 μg/mL, indicating low-toxicity of N,S-CDs. Notably, the bright blue fluorescence of N,S-CDs was quenched by introducing toxic Hg2+ ions into the solution. The detection limit was calculated to be about ∼3.5 nM, which is quite impressive compared to previous reports. Because of their low-toxicity, nano-size, and environment friendly properties, N,S-CDs could be excellent fluorescent agents for bio-imaging applications. The biological stability of fluorescent N,S-CDs was tested over time, and the findings were significant even after 8 days of incubation with T-ca cells. Because of good biocompatibility and bright fluorescence, N,S-CDs were suitable for in vivo imaging.