CD64
医学
流式细胞术
腹水
腹水
自发性细菌性腹膜炎
生物标志物
胃肠病学
微生物培养
内科学
败血症
免疫学
细菌
生物
遗传学
生物化学
作者
Elena González-López,Aitor Odriozola Herrán,Mónica Renuncio García,Adriel Roa-Bautista,Ángela Antón Rodríguez,Alejandra Comins-Boo,Juan Irure-Ventura,Ángela Puente,Marcos López-Hoyos,Jose Ignacio Fortea,David San Segundo
标识
DOI:10.1080/23744235.2023.2223294
摘要
Background CD64 expression on neutrophils surface (CD64N) by flow cytometry has been validated as a rapid biomarker for bacterial infections in both peripheral blood and other biological fluids. Ascites is a common complication in cirrhotic patients that a variety of factors can cause, including bacterial infections. Manual counting of polymorphonuclear (PMN) cells in ascitic fluid and microbiologic culture are essential for its diagnosis. We aimed to validate the determination of CD64N by flow cytometry in ascitic fluid and assess its potential usefulness in the rapid identification of bacterial infections.Materials and methods A prospective unicentre study was conducted. Flow cytometry was used to analyse the expression of CD64N in 77 ascitic fluid samples from the initial paracentesis of 60 cirrhotic patients in different admission episodes from November 2021 to December 2022.Results Seventeen samples were diagnosed with bacterial infection based on a positive microbiologic culture or by PMN count (>250 PMN/mm3 in ascitic fluid). The median of CD64N MFI was significantly increased in the bacterial infection group (3690.5 MFI [1635.23–6521.18] vs. 1105.9 MFI [737.3–2048.2], p < 0.001). The CD64 MFI ratio of granulocytes to lymphocytes was elevated in the bacterial infection group (13.06 [6.38–24.58] vs. 5.01 [3.38–7.36], p < 0.001). A CD64N ratio higher than 9.9 identified those patients with bacterial infection with 70.6 and 86.7% sensitivity and specificity, with an area under the curve (AUC) of 79.4%.Conclusion The CD64N determined by flow cytometry on ascitic fluid could help quickly identify bacterial infections in ascites patients, allowing early antibiotic treatment.
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