自噬
溶酶体
双光子激发显微术
体内
生物物理学
极性(国际关系)
细胞器
化学
荧光
细胞生物学
荧光寿命成像显微镜
生物化学
生物
酶
细胞
细胞凋亡
光学
物理
生物技术
作者
Baoshuai Wang,Longfang Ren,Tianyu Liang,Wei Hu,Qiang Tang
标识
DOI:10.1016/j.bios.2023.115453
摘要
As one of the important means for eukaryotic cells to maintain homeostasis, autophagy allows for transporting deformed biomacromolecules and damaged organelles to lysosome for digestion and degradation. The process of autophagy entails the merging of autophagosomes and lysosomes, culminating in the breakdown of biomacromolecules. This, in turn, leads to a change in lysosomal polarity. Therefore, fully understanding the changes of lysosomal polarity during autophagy is of significance to the study of membrane fluidity and enzymatic reaction. However, the shorter emission wavelength has greatly damaged the imaging depth, thus seriously limiting its biological application. Therefore, in this work, a near infrared in and out lysosome-targeted polarity-sensitive probe NCIC-Pola was developed. The fluorescence intensity of NCIC-Pola showed an approximate 1160-fold increase when the polarity decreased under two-photon excitation (TPE). In addition, the excellent fluorescence emission wavelength (692 nm) enabled the deep imaging analysis of scrap leather induced autophagy in vivo.
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