芹菜素
污渍
肌成纤维细胞
脊髓损伤
化学
纤维化
纤维连接蛋白
转化生长因子
脊髓
体内
病理
SMAD公司
细胞生物学
医学
生物
细胞外基质
生物化学
生物技术
精神科
抗氧化剂
基因
类黄酮
作者
Zhengxin Jin,Lijie Tian,Ying Zhang,Xiaodi Zhang,Jianning Kang,Hui Dong,Nana Huang,Liuzhu Pan,Bin Ning
摘要
To investigate the effect of apigenin on fibrous scar formation after mouse spinal cord injury (SCI).The pneumatic impactor strike method was used to establish an SCI model. Mice were intraperitoneally injected with 5 mg/kg or 20 mg/kg apigenin daily for 28 days after SCI. The Basso Mouse Scale (BMS) score, hematoxylin-eosin staining, and immunohistochemical staining were used to assess the effect of apigenin on scar formation and motor function recovery. Western blotting and qRT-PCR were used to detect the expression of fibrosis-related parameters in spinal cord tissue homogenates. NIH-3 T3 cells and mouse primary spinal cord fibroblasts, α-Smooth muscle actin (α-SMA), collagen 1, and fibronectin were used to evaluate apigenin's effect in vitro. Western blotting and immunofluorescence techniques were used to study the effect of apigenin on TGFβ/SMADs signaling.Apigenin inhibited fibrous scar formation in the mouse spinal cord and promoted the recovery of motor function. It reduced the expression of fibroblast-related parameters and increased the content of nerve growth factor in vivo, decreasing myofibroblast activation and collagen fiber formation by inhibiting TGFβ-induced SMAD2/3 phosphorylation and nuclear translocation in vitro.Apigenin inhibits fibrous scar formation after SCI by decreasing fibrosis-related factor expression through TGFβ/SMADs signaling.
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