Schsphenines A and B, two new lignans from the fruit of Schisandra sphenanthera with cardioprotective Activities

Schsphenines A (1) and B (2), two new lignans were isolated from the fruit of Schisandra sphenanthera, together with six known ones respectively identified as schisanhenol (3), methylgomisin O (4), wuweilignan E (5), neglschisandrin E (6), schisandrin A (7) and schisandrin B (8). The structures of isolated compounds were determined by UV, IR, HR-ESI-MS, NMR, ORD analysis and quantum chemical calculation. The in vitro cardioprotective activities of all compounds were studied on H₂O₂-injury H9C2 cells using the MTT method. Compound 1 exhibited a considerable protective effect with cell viability in 79.94 ± 2.36%. This research provided useful information for the utilisation of S. sphenanthera as the TCM.