纳米探针
适体
纳米片
化学
内吞作用
荧光
生物物理学
荧光寿命成像显微镜
费斯特共振能量转移
纳米载体
磁共振造影剂
细胞
纳米技术
纳米颗粒
分子生物学
钆
生物化学
材料科学
药物输送
有机化学
物理
生物
量子力学
作者
Zilong Zhao,Huanhuan Fan,Gaofeng Zhou,Huarong Bai,Hao Liang,Ruowen Wang,Xiaobing Zhang,Weihong Tan
摘要
A novel dual-activatable fluorescence/MRI bimodal platform is designed for tumor cell imaging by using a redoxable manganese dioxide (MnO2) nanosheet-aptamer nanoprobe. The redoxable MnO2 nanosheet acts as a DNA nanocarrier, fluorescence quencher, and intracellular glutathione (GSH)-activated MRI contrast agent. In the absence of target cells, neither fluorescence signaling nor MRI contrast of the nanoprobe is activated. In the presence of target cells, the binding of aptamers to their targets weakens the adsorption of aptamers on the MnO2 nanosheets, causing partial fluorescence recovery, illuminating the target cells, and also facilitating the endocytosis of nanoprobes into target cells. After endocytosis, the reduction of MnO2 nanosheets by GSH further activates the fluorescence signals and generates large amounts of Mn(2+) ions suitable for MRI. This platform should facilitate the development of various dual-activatable fluorescence/MRI bimodalities for use in cells or in vivo.
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