Anti-cancer effects of Kochia scoparia fruit in human breast cancer cells

Pharmacognosy Magazine,2014,10,39s,s661-s667.DOI:10.4103/0973-1296.139812Published:August 2014Type:Original ArticleAuthors:Hye-Yeon Han, Hyungwoo Kim, Yong Hae Son, Guemsan Lee, Sung-Hee Jeong, and Mi Heon Ryu Author(s) affiliations:Hye-Yeon Han1, Hyungwoo Kim2, Yong Hae Son2, Guemsan Lee3, Sung-Hee Jeong4, Mi Heon Ryu1 1 Department of Oral Pathology, School of Dentistry, Institute of Translational Dental Sciences, Gyeongnam, Korea 2 Division of Pharmacology, School of Korean Medicine, Pusan National University, Gyeongnam, Korea 3 Department of Herbology, College of Korean Medicine, Wonkwang University, Jeonbuk, Korea 4 Department of Oral Medicine, School of Dentistry, Dental Research Institute, Pusan National University, Gyeongnam, Korea Abstract:Background: The fruit of Kochia scoparia Scharder is widely used as a medicinal ingredient for the treatment of dysuria and skin diseases in China, Japan and Korea. Especially, K. scoparia had been used for breast masses and chest and flank pain. Objective: To investigate the anti-cancer effect of K. scoparia on breast cancer. Materials and Methods: We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in vitro. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, reactive oxygen species (ROS) generation and activation of apoptosis-associated proteins in MDA-MB-231, human breast cancer cells. Results: MTT assay results demonstrated that MEKS decreased the proliferation rates of MDA-MB-231 cells in a dose-dependent manner with an IC 50 value of 36.2 μg/ml. MEKS at 25 μg/ml significantly increased the sub-G1 DNA contents of MDA-MB-231 cells to 44.7%, versus untreated cells. In addition, MEKS induced apoptosis by increasing the levels of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP). Conclusion: These results suggest that MEKS inhibits cell proliferation and induces apoptosis in breast cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human breast cancer. Keywords:Apoptosis, Breast cancer, Cancer therapy, Kochia scoparia, reactive oxygen speciesView:PDF (2.15 MB) Full Text