细胞凋亡
癌症研究
乳腺摄影术
细胞培养
辐照
DNA损伤
程序性细胞死亡
细胞
体外
医学
恶性肿瘤
核医学
化学
病理
乳腺癌
生物
癌症
内科学
DNA
遗传学
物理
生物化学
核物理学
作者
L.C. Pereira,Marcella T. Ferreira,Antonio Gilclêr Ferreira Lima,Camila Salata,Samara Cristina Ferreira-Machado,Ildercílio Mota de Souza Lima,Verônica Morandi,Luís Alexandre Gonçalves Magalhães
标识
DOI:10.1016/j.ejmp.2021.06.002
摘要
PurposeMammography is the diagnostic imaging practice used in screening to detect early lesions suspected of malignancy. It uses a low energy X-ray beam in which a low dose in the order of 2–3 mGy is delivered to patient breast cells. However, it has been speculated that it could lead to significant cell damage, when compared to conventional X-ray. We investigated the biological effects of low doses, with mean glandular doses (MGDs) of 2.5 mGy and 2.5 + 2.5 mGy, on mammary cells in vitro.MethodsWe used the non-tumorigenic cell line (MCF-10A) and two tumor cells lines (MCF-7 and MDA-MB-231). Colony formation, apoptosis, and double-strand DNA breaks (DSBs) were quantified.ResultsThe selected MGD regimens did not alter the formation of colonies by any of the cell lines. MCF-7 cells exhibited a markedly increase in apoptosis, 24 h after the single-dose protocol; MCF-10A cells underwent apoptosis only after 72 h, with both irradiation regimens, while MDA-MB-231 cells (highly invasive and metastatic) were not susceptible to apoptosis. The detection of γH2AX histone in the nuclei of irradiated cells showed that the double-dose resulted in increase of DSBs, especially in tumor cell lines.ConclusionsAlthough the health benefits of early breast screening remain indisputable, our future perspective is to better understand the biological basis for the effects of low dose radiation on breast cells and to investigate if and under what conditions there would be a risky situation in repeated mammography screening, in both asymptomatic and symptomatic women.
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