作者
Tao Tan,Jun Wu,Chenyang Si,Shao‐Xing Dai,Youyue Zhang,Nianqin Sun,E Zhang,Honglian Shao,Wei Si,Pengpeng Yang,Hong Wang,Zhenzhen Chen,Ran Zhu,Yu Kang,Reyna Hernández-Benítez,Llanos Martinez Martinez,Estrella Núñez-Delicado,W. Travis Berggren,May Schwarz,Zongyong Ai,Tianqing Li,Hongkui Deng,Concepción Rodrı́guez Esteban,Weizhi Ji,Yuyu Niu,Juan Carlos Izpisúa Belmonte
摘要
Interspecies chimera formation with human pluripotent stem cells (hPSCs) represents a necessary alternative to evaluate hPSC pluripotency in vivo and might constitute a promising strategy for various regenerative medicine applications, including the generation of organs and tissues for transplantation. Studies using mouse and pig embryos suggest that hPSCs do not robustly contribute to chimera formation in species evolutionarily distant to humans. We studied the chimeric competency of human extended pluripotent stem cells (hEPSCs) in cynomolgus monkey (Macaca fascicularis) embryos cultured ex vivo. We demonstrate that hEPSCs survived, proliferated, and generated several peri- and early post-implantation cell lineages inside monkey embryos. We also uncovered signaling events underlying interspecific crosstalk that may help shape the unique developmental trajectories of human and monkey cells within chimeric embryos. These results may help to better understand early human development and primate evolution and develop strategies to improve human chimerism in evolutionarily distant species.